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Position：Dib ® Probe qPCR SuperMix Plus
■ Product Description:
This product is a fluorescent probe (TaqMan or Molecular Beacon, etc.) added to the PCR system. In the amplification process, the amount of fluorescence
A kit that is proportional to the amount of amplification products and determines the amount of nucleic acid of samples by fluorescence. The new process has been modified in the kit
HotStart Taq DNA polymerase, the optimal reaction Buffer for qPCR optimization, dNTPs, PCR stabilizers and enhancers and other reagents were premiered
Together, it's a 2 x premixed reagent.
HotStart Taq DNA polymerase modified by the new technology can effectively inhibit non-specific amplification at low temperature, and significantly improve the amplification efficiency and
Sensitivity, can be in a wider range of accurate quantification. Especially for some special templates, such as African swine fever virus, the effect is better. try
ROX Reference Dye with different concentrations is equipped in the reagent box to correct the fluorescence signal error between holes with different instruments.
■ Product features:
High specificity: Antibody modified HotStart Taq DNA polymerase is prepared by a novel process, which can perform PCR reaction without heat start-up.
Greatly improve the specificity of PCR amplification.
High efficiency: DiB carefully formulated RealTime PCR special 2×SuperMix for higher amplification efficiency and sensitivity.
Fast: the reagents necessary for PCR reaction are collected in one tube, and the reaction system can be prepared in a few minutes.
■ Suggestions:
When using, please mix gently and evenly upside down to avoid foaming, and use after slight centrifugation. Please use new solution for preparation and packaging
The (non-polluting) head, Microtube, etc., avoid pollution.
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Save:
Store at -20℃ away from light. If you need to take it frequently in a period of time, it can be stored at 2~8℃ for 3 months. Avoid repeated freezing and thawing.
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Quality Assurance:
Purity test: all components were tested without endonuclease, exonuclease and nucleic acid residues.
■ Product packaging:
The component of product
A packaging
B packing
2× DIB ® Probe qPCR SuperMix Plus
1 ml x 5
1 ml x 25
RNase Free Water
1 ml x 5
1 ml x 23
Reference Dye I (ROX I)
200 mu l
1ml
ROX Reference Dye II
200 mu l
1mlHong Kong ABC Biotech Co.,Limited
Room 502C,5th floor, Happy View Commercial Centre,
2-6 Garden Street,Mongkok, Kowloon, Hong Kong
ROX I calibrated real-time PCR apparatus includes:
ABI 5700/7000/7300/7700/7900/7900 HT/7900 HT Fast; ABI StepOne/StepOnePlus and other instruments.
Real Time PCR instruments calibrated with ROX II include:
ABI 7500/7500 Fast; ABI ViiA7; ABI Q5 Q6;
ABI Quant Studio 6/7 Flex; Stratagene MX4000/MX3500P/MX3000P and other instruments.
Real Time PCR machines that do not require ROX calibration include:
Bio - Rad CFX96 provided/CFX 384 / iCycler iQ5 / / My iQ iQ5 MiniOpticon/an Opticon/an Opticon/Chromo4; Eppendorf
MasterCyclerrealplex/realplex 2 s.
Cepheid SmartCycler; Illumina Eco qPCR.
Roche Applied Science LightCycler 480;
Thermo Scientific PikoReal Cycler;
Qiagen/Corbett rotor-gene Q/ rotor-gene 3000/ rotor-gene 6000 and other instruments.
■ Operation method:
Common reaction system (20μ L) :
2× DIB ® Probe qPCR SuperMix Plus
10 mu l
The upstream primer
0.2-1.0μM (final concentration)
Downstream primers
0.2-1.0μM (final concentration)
The template
T
aq
1-2 mu l
ROX (depending on machine model)
0.4 u l
RNase Free Water
To 20 mu l
Common PCR cycles * :
* High specificity can be obtained by two-step method and high amplification rate can be obtained by three-step method.
Two-step amplification program:
94 ℃,
1min
35-45 times cycle
95 ℃ and 20 s
60 ℃, 30 s
Three-step PCR amplification procedure:
94 ℃,
1min
95 ℃ and 20 s
35-45 times cycle
50 to 60 ℃ and 20 s
72 ℃, 30 s